In Jainism, there is no canonical teaching that a mala must have exactly 27 knots. The knots in a knotted mala are mainly practical: they help separate beads to protect the cord from wear and make counting of mantras easier. The specific idea of “27 knots” does not form a standard Jain scriptural significance.

What is clearer in Jain tradition is the idea of sacred counts and auspicious numbers in related contexts:

• Jains speak of the 27 bhavs (births/life phases) of Mahavira in his prior lives, which is a doctrinal detail about his past lives, not about malas. ( jainknowledge.com)
• The 27 gathas (verses) of the Uvasaggaharam Stotra (a well-known Jain prayer) is a separate, scriptural composition, not a knotting rule for malas. ( jainknowledge.com)
• Some Jain texts or stotras (e.g., the Ajit Shanti Sutra excerpt) show verses and ritual phrases, but again, these do not prescribe a universal 27-knot mala rule. ( jainknowledge.com)

If you are asking about a specific Jain practice you’ve heard of, there might be a local or tradition-specific custom, but it is not a universal Jain standard.