54th letter of navakar mantra
Navkar mantra has 68 akshar (letters) in the traditional counting. The 54th akshar falls in the later part (the Chulika). In that sequence, the 54th letter corresponds to the guru-letter “vve” (spoken as vva/vve in some recitations). This is one of the letters classified as a guru in the Chulika section.
You can read more about how the Navkar mantra is counted and its akshar distribution here